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Biological Indicator for Steam Sterilization is a Paper Carrier individually packaged in a suitable container readily penetrable by steam and designed to hold an appropriate bacteriological culture medium, so as to enable the packaged carrier after subjection to saturated steam sterilization conditions to be incubated in the supplied medium in a self-contained system. The supplied medium may contain a suitable indicator as a convenience for determining by a color change whether or not spores have survived. The design of the self-contained system is such that, after exposure to the specified sterilization conditions and inoculation of the medium under closed conditions as stated in the labeling, there is no loss of medium and inoculate during subsequent transport and handling, if done according to the provided instructions. The materials of which the self-contained system are made are such that there is no retention or release of any substance that may cause inhibition of growth of surviving spores under the incubation conditions stated in the labeling.