Module C: Advanced Techniques

(A)INTRODUCTION & PRINCIPLES of HPLC - G.C. AND THEIR COMPONENTS. The various Modes of chromatographic separation(Reversed phase, Normal phase etc.).

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(A)INTRODUCTION & PRINCIPLES of HPLC - G.C. AND THEIR COMPONENTS.

The various Modes of chromatographic separation(Reversed phase, Normal phase etc.)

Understanding working of HPLC, degassing the mobile phase and preparation of sample.

To control retention and selectivity in reversed-phase LC.Concept of gradient elution

Analytical method validation and troubleshooting

Introduction to gas chromatography(GC). Column types and selection. Sample introduction techniques

Reviewing , liquid, and other injection methods. FID detection systems

Analysis and interpretation of results. Routine maintenance and troubleshooting

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